RNA Modifications in Health and Disease

RNA molecules are frequently modified post-transcriptionally, and these modifications required for proper functions. To date, about 150 different types of chemical have been identified in various across all domains life. There still a number novel to be discovered. appear confer diversities simple basically composed four letters, acquire greater variety biological These play critical roles stability functions molecules. We've carrying out project identify from sources, reported seven so far. Taking advantage mass spectrometric analysis modifications, we systematically screened series knockout strains uncharacterized genes, more than 40 genes responsible tRNA rRNA as well mRNA modification. Recently, cap-specific adenosine methyltransferase (CAPAM) N6-methylation m6Am at the transcription start site eukaryotic mRNAs. It has generally thought that stable static, their frequencies rarely regulated. We previously lack modification causes major classes mitochondrial diseases including MELAS MERRF. Deficient results defective protein synthesis, leading dysfunction. findings provided first evidence human disease caused by an disorder. call “RNA modopathy” new category diseases. I am going show our recent studies on associated with dynamic regulation sensing intracellular metabolites under physiological condition.